Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using PDK3(GTX104286) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HepG2, using PDK3(GTX104286) antibody at 1:200 dilution.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.