Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB3 antibody [C3], C-term (GTX108335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of PFKFB3 protein from A431 whole cell extracts using 5 μg of PFKFB3 antibody [C3], C-term (GTX108335).
Western blot analysis was performed using PFKFB3 antibody [C3], C-term (GTX108335).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

PFKFB3 antibody [C3], C-term detects PFKFB3 protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
PFKFB3 antibody [C3], C-term (GTX108335) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min