Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody (GTX107755) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

PFKFB4 antibody detects PFKFB4 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PFKFB4 antibody (GTX107755) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PFKFB4 antibody detects PFKFB4 protein at cytoplasm on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
PFKFB4 antibody (GTX107755) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PFKFB4 antibody detects PFKFB4 protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: PFKFB4 protein stained by PFKFB4 antibody (GTX107755) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PFKFB4(GTX107755) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PFKFB4 antibody detects PFKFB4 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody (GTX107755) diluted at 1:2000.