PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGD protein stained by PGD antibody [N1N3] (GTX101703) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (GTX101703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PGD antibody [N1N3] detects PGD protein at cytoplasm on A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
PGD antibody [N1N3] (GTX101703) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg whole cell lysate)
A:293T
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
10% SDS PAGE
GTX101703 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PGD protein stained by PGD antibody [N1N3] (GTX101703) diluted at 1:500.
Blue: Hoechst 33342 staining.

PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (GTX101703) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal PLoS Biol in 2010.PMID: 21049082