Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [N1C1] (GTX101405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PGK1 antibody [N1C1] detects PGK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PGK1 stained by PGK1 antibody [N1C1] (GTX101405) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101405 diluted at 1:1000

PGK1 antibody [N1C1] detects PGK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PGK1 stained by PGK1 antibody [N1C1] (GTX101405) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
10% SDS PAGE
GTX101405 diluted at 1:1000