Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody [HL3391] (GTX641211) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody [HL3391] (GTX641211) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.