PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

PGP9.5 antibody detects PGP9.5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PGP9.5 antibody (GTX109637) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PGP9.5 antibody detects PGP9.5 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:5000

PGP9.5 antibody detects PGP9.5 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
12% SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:10000.

PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded rat colon.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse skin.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 stained by PGP9.5 antibody (GTX109637) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

The data was published in the journal Cells in 2020. PMID: 32155948