PHF10 antibody detects PHF10 protein at nucleus in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
PHF10 antibody (GTX116314) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PHF10 antibody detects PHF10 protein by western blot analysis.
A.50 μg mouse colon lysate/extract
10% SDS-PAGE
PHF10 antibody (GTX116314) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PHF10 antibody detects PHF10 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PHF10 protein stained by PHF10 antibody (GTX116314) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PHF10(GTX116314) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PHF10 antibody detects PHF10 protein at nucleus in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
PHF10 antibody (GTX116314) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF10 antibody (GTX116314) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.