Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PHGDH antibody [N1N2], N-term detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using PHGDH(GTX101948) antibody at 1:500 dilution.

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101948 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101948 diluted at 1:1000

PHGDH antibody [N1N2], N-term detects PHGDH protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: PHGDH stained by PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PHGDH(GTX101948) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term (GTX101948).
Western blot analysis was performed using PHGDH antibody [N1N2], N-term (GTX101948).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.