PI3 kinase C2 alpha antibody [HL1612] detects PI3 kinase C2 alpha protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PI3 kinase C2 alpha stained by PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.