Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody (GTX100462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody (GTX100462) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: C2C12
B: C2C12+AEA10mM
GTX100462 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Human Breast Cancer, using PI3 kinase p110 alpha(GTX100462) antibody.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PI3 kinase p110 alpha antibody detects PI3 kinase p110 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody (GTX100462) diluted at 1:500.
Blue: Hoechst 33342 staining.