Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody (GTX132597) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody (GTX132597) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.