Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI4 kinase alpha antibody [C1C2], Internal (GTX107441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PI4 kinase alpha(GTX107441) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PI4 kinase alpha antibody [C1C2], Internal detects PI4 kinase alpha protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PI4 kinase alpha protein stained by PI4 kinase alpha antibody [C1C2], Internal (GTX107441) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.