Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PIKfyve antibody [HL3510] (GTX641398) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.