Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PINK1 antibody [N3C3] (GTX107851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (CCCP: carbonyl cyanide 3-chlorophenylhydrazone)

PINK1 antibody [N3C3] detects PINK1 protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: PINK1 protein stained by PINK1 antibody [N3C3] (GTX107851) diluted at 1:500.
Blue: Hoechst 33342 staining.

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX107851 diluted at 1:1000

The data was published in the 2020 in Int J Mol Sci. PMID: 33260618