PIP5K1A antibody [HL2011] detects PIP5K1A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat spleen.
PIP5K1A stained by PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PIP5K1A antibody [HL2011] detects PIP5K1A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PIP5K1A stained by PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.