WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25798 PKC eta (phospho Thr655) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptide corresponding to the immunogen from other PKC isoforms (Lane 5-9). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 10) eliminates the signal further verifying that the antibody is phospho-specific.
Dilution : 1:2000