Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC zeta antibody (GTX111598) diluted at 1:1000.

PKC zeta antibody detects PKC zeta protein at cytoplasm on SW480 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SW480 xenograft.
PKC zeta antibody (GTX111598) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: zebrafish brain
7.5% SDS PAGE
GTX111598 diluted at 1:500

The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23840253