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PKM antibody [GT1810]

Anti-PKM antibody [GT1810] used in Western Blot (WB). GTX634796
Anti-PKM antibody [GT1810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634796
Anti-PKM antibody [GT1810] used in Western Blot (WB). GTX634796
Anti-PKM antibody [GT1810] used in IHC (Paraffin sections) (IHC-P). GTX634796

Cat. No. GTX634796

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1810

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PKM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKM. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888477

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate kinase M1/2 , CTHBP , HEL-S-30 , OIP3 , PK3 , PKM2 , TCB , THBP1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-PKM antibody [GT1810] used in Western Blot (WB). GTX634796

GTX634796 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody [GT1810] (GTX634796) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PKM antibody [GT1810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634796

GTX634796 ICC/IF Image

PKM antibody [GT1810] detects PKM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKM stained by PKM antibody [GT1810] (GTX634796) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-PKM antibody [GT1810] used in Western Blot (WB). GTX634796

GTX634796 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody [GT1810] (GTX634796) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PKM antibody [GT1810] used in IHC (Paraffin sections) (IHC-P). GTX634796

GTX634796 IHC-P Image

PKM antibody [GT1810] detects PKM protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PKM stained by PKM antibody [GT1810] (GTX634796) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations