PKM antibody [GT1810] detects PKM protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PKM stained by PKM antibody [GT1810] (GTX634796) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody [GT1810] (GTX634796) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

PKM antibody [GT1810] detects PKM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKM stained by PKM antibody [GT1810] (GTX634796) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody [GT1810] (GTX634796) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.