Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500.

Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.
A. 50 μg Rat brain lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (muscle) antibody (GTX107681) dilution: 1:1000

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PKM antibody detects PKM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKM stained by PKM antibody (GTX107681) diluted at 1:500.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX107681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

PKM antibody detects PKM protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PKM stained by PKM antibody (GTX107681) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min