Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111319) diluted at 1:500.

Pyruvate Kinase (muscle) antibody detects PKM2 protein by Western blot analysis.
A. 50 μg Rat muscle lysate/extract
7.5 % SDS-PAGE
Pyruvate Kinase (muscle) antibody (GTX111319) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX111319 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111319 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase(muscle)(GTX111319) antibody at 1:100 dilution.