GeneTex
PKM antibody
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111604) diluted at 1:10000.
PKM antibody detects PKM protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PKM stained by PKM antibody (GTX111604) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PKM antibody detects PKM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKM protein stained by PKM antibody (GTX111604) diluted at 1:500.
Blue: Hoechst 33342 staining.
Pyruvate Kinase (muscle) antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Pyruvate Kinase (muscle) antibody (GTX111604) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody (GTX111604) diluted at 1:5000.
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX111604 diluted at 1:5000
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HostRabbit
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ClonalityPolyclonal
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IsotypeIgG
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ApplicationsWB ICC/IF IHC-P
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ReactivityHuman, Mouse, Rat