Untreated (–) and treated (+) Raji whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG2 (phospho Tyr1217) antibody [HL3406] (GTX641243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.