Untreated (–) and treated (+) Raji whole cell extract (10 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLCG2 (phospho Tyr1217) antibody (GTX133610) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (10 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG2 (phospho Tyr1217) antibody (GTX133610) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.