PLK1 antibody [N2C2], Internal detects PLK1 protein at midbody by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformadehyde at RT for 30 min.
Green: PLK1 protein stained by PLK1 antibody [N2C2], Internal (GTX104302) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLK1 antibody [N2C2], Internal (GTX104302) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human skin, using PLK1(GTX104302) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLK1 antibody [N2C2], Internal (GTX104302) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.