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PLK1 antibody

Anti-PLK1 antibody used in Western Blot (WB). GTX54548
Anti-PLK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54548
Anti-PLK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54548
Anti-PLK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54548

Cat. No. GTX54548

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PLK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 304-603 of human PLK1 (NP_005021.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polo like kinase 1 , PLK , STPK13

Cellular Localization

Nucleus

Background

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PLK1 antibody used in Western Blot (WB). GTX54548

GTX54548 WB Image

WB analysis of various sample lysates using GTX54548 PLK1 antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-PLK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54548

GTX54548 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54548 PLK1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PLK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54548

GTX54548 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54548 PLK1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PLK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54548

GTX54548 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54548 PLK1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399