ICC/IF analysis of U251 cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

WB analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3) lysates using GTX22825 PMCA ATPase antibody [5F10].
Dilution : 1:2000

IHC-P analysis of human colon carcinoma tissue using GTX22825 PMCA ATPase antibody [5F10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

IHC-P analysis of human brain tissue using GTX22825 PMCA ATPase antibody [5F10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

ICC/IF analysis of C6 cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

FACS analysis of Jurkat cells using GTX22825 PMCA ATPase antibody [5F10] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of HeLa cells using GTX22825 PMCA ATPase antibody [5F10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

FACS analysis of Hela cells using GTX22825 PMCA ATPase antibody [5F10] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

IHC-P analysis of human tonsil tissue using GTX22825 PMCA ATPase antibody [5F10].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200