Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PNMT antibody [N2C3] (GTX114098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PNMT antibody [N2C3] (GTX114098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PNMT antibody [N2C3] (GTX114098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PNMT(GTX114098) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PNMT antibody [N2C3] detects PNMT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
PNMT stained by PNMT antibody [N2C3] (GTX114098) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min