Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using POM121(GTX102128) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11305 (Red) at 1:500.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with POM121 antibody [N2N3] (GTX102128) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using POM121(GTX102128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using POM121(GTX102128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal Acta Neuropathol in 2016. PMID: 26839082