IHC-P analysis of human lung cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

IHC-P analysis of human lung cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

IHC-P analysis of human brain tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

IHC-P analysis of human colorectal cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

IHC-P analysis of human normal tissues adjacent to esophageal cancer using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX02531 PPAR gamma (phospho Ser112) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

IHC-P analysis of human mammary cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

IHC-P analysis of human mammary cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

IHC-P analysis of human colorectal cancer tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

ICC/IF analysis of PFA-fixed HepG2 cells (30min of 4μM Forskolin treatment) using GTX02531 PPAR gamma (phospho Ser112) antibody.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

ICC/IF analysis of PFA-fixed HeLa cells using GTX02531 PPAR gamma (phospho Ser112) antibody.
Permeabilization : 0.1% saponin
Dilution : 1:400

WB analysis of extracts from mouse liver and rat lung tissue using GTX02531 PPAR gamma (phospho Ser112) antibody.
N peptide : Non-phospho peptide
P peptide : Phospho peptide