Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPAT antibody (GTX102725) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of methanol-fixed HeLa, using PPAT(GTX102725) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
7.5% SDS PAGE
GTX102725 diluted at 1:1000