Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPM1A antibody (GTX111288) diluted at 1:500.

PPM1A antibody detects PPM1A protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: PPM1A protein stained by PPM1A antibody (GTX111288) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPM1A(GTX111288) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX111288 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
10% SDS PAGE
GTX111288 diluted at 1:1000