WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using GTX105255 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) GTX70220 at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PPP1A antibody detects PPP1A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PPP1A stained by PPP1A antibody (GTX105255) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A(GTX105255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP1A(GTX105255) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PPP1A antibody (GTX105255) at 1:300 dilution.

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted at 1:500.