Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Thr246) antibody (GTX133176) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH3T3 whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Thr246) antibody (GTX133176) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Thr246) antibody (GTX133176) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.