Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX116478 diluted at 1:5000

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX116478 diluted at 1:1000

PRMT3 antibody immunoprecipitates PRMT3 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of PRMT3 protein by 4 μg PRMT3 antibody (GTX116478)
7.5 % SDS-PAGE
The immunoprecipitated PRMT3 protein was detected by PRMT3 antibody (GTX116478) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT3 antibody (GTX116478) diluted at 1:1000.