Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [N3C3] (GTX115409) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PRMT5 antibody [N3C3] detects PRMT5 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PRMT5 antibody [N3C3] (GTX115409) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PRMT5 antibody [N3C3] detects PRMT5 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PRMT5 stained by PRMT5 antibody [N3C3] (GTX115409) diluted at 1:2500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PRMT5 antibody [N3C3] detects PRMT5 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PRMT5 antibody [N3C3] (GTX115409) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded Mahlavu xenograft, using PRMT5(GTX115409) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PRMT5 antibody [N3C3] detects PRMT5 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRMT5 stained by PRMT5 antibody [N3C3] (GTX115409) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.