Wild-type (WT) and PRX III knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX III antibody [N1C3] (GTX112004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Peroxiredoxin 3(GTX112004) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Peroxiredoxin 3 antibody [N1C3] detects Peroxiredoxin 3 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [N1C3] (GTX112004) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
C: Molt-4 (GTX27912)
D: Raji
12% SDS PAGE
GTX112004 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112004 diluted at 1:1000