Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] (GTX633629) diluted at 1:500.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] (GTX633629) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] (GTX633629) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

PSAT1 antibody [GT1353] detects PSAT1 protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSAT1 stained by PSAT1 antibody [GT1353] (GTX633629) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

PSAT1 antibody [GT1353] detects PSAT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSAT1 protein stained by PSAT1 antibody [GT1353] (GTX633629) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

PSAT1 antibody [GT1353] detects secreted PSAT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PSAT1 stained by PSAT1 antibody [GT1353] (GTX633629) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min