Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using proteasome alpha 7(GTX101745) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMA7 antibody [N1C3] (GTX101745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
12% SDS PAGE
GTX101745 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101745 diluted at 1:1000