Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME1 antibody (GTX118374) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

PSME1 antibody detects PSME1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSME1 protein stained by PSME1 antibody (GTX118374) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.