Wild-type (WT) and PTS knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PTS antibody [N1C3] (GTX110549) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PTS(GTX110549) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using PTS(GTX110549) antibody at 1:100 dilution.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PTS antibody [N1C3] (GTX110549) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: H1299
15% SDS PAGE
GTX110549 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse stomach
15% SDS PAGE
GTX110549 diluted at 1:1000

The data was published in the journal Hum Mol Genet in 2016.PMID: 27798097