Sample (30 ug of whole cell lysate)
A: HCT116 cells with mock treatment for 0hr
B: HCT116 cells with 30uM cisplatin treatment for 24hr
15% SDS PAGE
GTX109675 diluted at 1:1000

PUMA antibody [C2C3], C-term immunoprecipitates BCL2 binding component 3 protein in IP experiments.
IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 24 hr
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL2 binding component 3 protein by 4 μg PUMA antibody [C2C3], C-term (GTX109675)
15 % SDS-PAGE
The immunoprecipitated BCL2 binding component 3 protein was detected by PUMA antibody [C2C3], C-term (GTX109675) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PUMA(GTX109675) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PUMA antibody detects PUMA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12 % SDS-PAGE, and blotted with PUMA antibody (GTX109675) diluted by 1:500