Pancreatic Polypeptide antibody [GT6512]

Cat. No. GTX628832

Host	Mouse
Clonality	Monoclonal
Clone Name	GT6512
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Pancreatic Polypeptide products

Datasheet
Document

View product citations for antibody GTX628832 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Positive Control

human PPY-transfected 293T cells

Predict Reactivity

Bovine, Dog, Guinea pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Pancreatic polypeptide. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888058

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pancreatic polypeptide , PNP , PP

Cellular Localization

Secreted

Background

This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Database

Gene ID: 5539 PPY
UniProt: P01298 PPY

Research Area

DATA IMAGES

GTX628832 IHC-P Image

Pancreatic Polypeptide antibody [GT6512] detects Pancreatic Polypeptide protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Pancreatic Polypeptide stained by Pancreatic Polypeptide antibody [GT6512] (GTX628832) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX628832 WB Image

Pancreatic polypeptide antibody [GT6512] detects PPY protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug whole cell lysate/extract of human PPY-transfected 293T cells
15 % SDS-PAGE
Pancreatic polypeptide antibody [GT6512] (GTX628832) dilution: 1:5000

GTX628832 ICC/IF Image

Pancreatic Polypeptide antibody [GT6512] detects Pancreatic Polypeptide protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pancreatic Polypeptide stained by Pancreatic Polypeptide antibody [GT6512] (GTX628832) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations