Pepsinogen I antibody [HL2137] detects Pepsinogen I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human stomach.
Pepsinogen I stained by Pepsinogen I antibody [HL2137] (GTX638109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Indirect ELISA analysis was performed by coating the plate with recombinant NS0 cells expressed, full-length human pepsinogen A protein (97.56-1.52 nM). Coated protein was probed with Pepsinogen I antibody [HL2137] (GTX638109) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Pepsinogen I antibody [HL2137] detects Pepsinogen I protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pepsinogen I stained by Pepsinogen I antibody [HL2137] (GTX638109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pepsinogen I antibody [HL2137] (GTX638109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pepsinogen I antibody [HL2137] (GTX638109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.