Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban antibody (GTX109254) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using phospholamban(GTX109254) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min