Untreated (–) and treated (+) Mouse tissue extract (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban (phospho Ser16) antibody [HL1710] (GTX637309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.