Untreated (–) and treated (+) Mouse tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Phospholamban (phospho Ser16) antibody [HL3794] (GTX642013) diluted at 1:1000 and Phospholamban antibody (GTX109254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.