ICC/IF analysis of HeLa cells using GTX25592 Phosphotyrosine antibody [IG2].
Green : Primary antibody

IP analysis of K562 cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours using GTX25592 Phosphotyrosine antibody [IG2].
IP antibody:GTX25592
IB antibody : c-abl antibody
IP reaction : 2μg antibody / 500μg lysate

Tyrosine phosphorylated c-Abl and Bcr-Abl was performed following immunoprecipitation of K562 cell lysates using a c-Abl antibody, from untreated cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours. WB was performed using GTX25592 Phosphotyrosine antibody [IG2] at a dilution of 1:250.