APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Note :
WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
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Not tested in other applications.
Calculated MW
Positive Control
A431 , Unboiled U87-MG
Predict Reactivity
Mouse, Rat, Bovine, Cat, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.46 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PiT2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951923
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
solute carrier family 20 member 2 , GLVR-2 , GLVR2 , IBGC1 , IBGC3 , MLVAR , PIT-2 , PIT2 , RAM1 , Ram-1
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
Sodium-phosphate symporter which seems to play a fundamental housekeeping role in phosphate transport by absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. In vitro, sodium-dependent phosphate uptake is not siginificantly affected by acidic and alkaline conditions, however sodium-independent phosphate uptake occurs at acidic conditions. May play a role in extracellular matrix, cartilage and vascular calcification. Functions as a retroviral receptor and confers human cells susceptibility to infection to amphotropic murine leukemia virus (A-MuLV), 10A1 murine leukemia virus (10A1 MLV) and some feline leukemia virus subgroup B (FeLV-B) variants.
Database
Research Area
DATA IMAGES
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GTX105078 WB Image
A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PiT2 antibody [N2C1], Internal (GTX105078) diluted at 1:500.
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GTX105078 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PiT2 antibody [N2C1], Internal (GTX105078) diluted at 1:500.
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REFERENCE
There are currently no references for PiT2 antibody [N2C1], Internal (GTX105078). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PiT2 antibody [N2C1], Internal (GTX105078). Be the first to share your experience with this product.