Immunofluorescence analysis of methanol-fixed HeLa, using Plexin D1(GTX104765) antibody at 1:500 dilution.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Plexin D1 antibody [C3], C-term (GTX104765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.